Functional Evidence for Indirect Recognition of G·U in tRNA Ala by Alanyl-tRNA Synthetase
- 12 January 1996
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 271 (5246) , 195-197
- https://doi.org/10.1126/science.271.5246.195
Abstract
The structural features of the G⋅U wobble pair in Escherichia coli alanine transfer RNA (tRNA Ala ) that are associated with aminoacylation by alanyl-tRNA synthetase (AlaRS) were investigated in vivo for wild-type tRNA Ala and mutant tRNAs with G⋅U substitutions. tRNA Ala with G⋅U, C⋅A, or G⋅A gave similar amounts of charged tRNA Ala and supported viability of E. coli lacking chromosomal tRNA Ala genes. tRNA Ala with G⋅C was inactive. Recognition of G⋅U by AlaRS thus requires more than the functional groups on G⋅U in a regular helix and may involve detection of a helical distortion.Keywords
This publication has 19 references indexed in Scilit:
- Structure of the P1 Helix from Group I Self-splicing IntronsJournal of Molecular Biology, 1995
- Minor Groove Recognition of the Conserved G⋅U Pair at the Tetrahymena ribozyme Reaction SiteScience, 1995
- tRNA Structure and Aminoacylation EfficiencyProgress in Nucleic Acid Research and Molecular Biology, 1993
- Specificity for Aminoacylation of an RNA Helix: an Unpaired, Exocyclic Amino Group in the Minor GrooveScience, 1991
- Precise mapping and comparison of two evolutionarily related regions of the Escherichia coli K-12 chromosomeJournal of Molecular Biology, 1990
- Genomic organization and physical mapping of the transfer RNA genes in Escherichia coli K12Journal of Molecular Biology, 1990
- Association of Transfer RNA Acceptor Identity with a Helical IrregularityScience, 1988
- Nucleotides that contribute to the identity of Escherichia coli tRNAPheJournal of Molecular Biology, 1988
- A simple structural feature is a major determinant of the identity of a transfer RNANature, 1988
- Changing the Identity of a tRNA by Introducing a G-U Wobble Pair Near the 3′ Acceptor EndScience, 1988