In vitro Translation of Beet Necrotic Yellow Vein Virus RNA and Studies of Sequence Homology among the RNA Species Using Cloned cDNA Probes

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Abstract
SUMMARY The four RNA species of a French strain of beet necrotic yellow vein virus (BNYVV) have lengths of 7100, 4800, 1800 and 1500 nucleotides, as measured by agarose gel electrophoresis in denaturing conditions. Translation of purified RNA in a reticulocyte cell-free system revealed that RNA-2 encodes the 22000 mol. wt. coat protein. Cloned cDNA copies of each of the four viral RNA species have been used to show that BNYVV RNA-3 and RNA-4 share no sequence homology with RNA-1 and RNA-2. Thus, RNA-3 and RNA-4 cannot be subgenomic messengers derived from the larger RNA species. Beet necrotic yellow vein virus (BNYVV) is a soil-borne virus that causes a severe disease of sugar beet (Beta vulgaris) known as rhizomania (Tamada & Baba, 1973). The vector of the virus is the plasmodiophoromycete fungus Polymyxa betae (Tamada, 1975; Fujisawa & Sugimoto, 1976), which is responsible for persistence of the virus in infected soil. BNYVV has rigid rod- shaped virions which fall into four distinct length classes: 390 nm, 265 rim, 100 nm and 85 nm for a French isolate (Putz, 1977). The virions contain four species of single-stranded RNA with reported molecular weights of 2.3 x 10 6, 1.8 × 10 6, 0"7 × 106 and 0.6 x 106 which are roughly in proportion to the lengths of the four classes of virion (Putz, 1977). In this paper we have studied the translation of BNYVV RNA in a cell-free system and investigated the sequence

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