Microsomal Ca2+‐ and phospholipid‐dependent protein kinase
- 13 June 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 156 (2) , 231-234
- https://doi.org/10.1016/0014-5793(83)80502-x
Abstract
A Ca2+- and phospholipid-dependent protein kinase (CaPK) has been identified in rat liver microsomes. CaPK isolated from liver cytosol bound to smooth microsomes in the presence of 100 μM CaCl2. A saturation in binding was observed when a 5-fold excess of enzyme over that present in microsomes had become bound. The microsomal CaPK and 50% of the enzyme bound in vitro was not removed by EGTA treatment. This suggests that Ca2+ is required for the binding of CaPK to microsomes, but not for the retention of the enzyme on the membrane.Keywords
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