A Micro-Colony-Inhibition Method for Quantitation of Tumor Immunity 2

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Abstract
A new micro-colony-inhibition system has been designed to quantitate syngeneic and allogeneic immunity against tumor-associated antigens and to compare the antigenicity of different tumor cells. It is relatively simple to use and appears to be at least as sensitive as the chromium-release method. We previously showedthat the acrylamide surface is excellent for growing tumor cells at low densities and appears to be amenable to use with most tumor cells. This new micro-colony-inhibition system uses an extremely small number of target cells, apparently considerably lower than that employed by any of the other cellular immunity assays. Syngeneic immunity against tumor-associated antigens was demonstrated by preincubation of spleen cells from either immunized or tumor-bearing animals.