A U1/U4/U5 snRNP Complex Induced by a 2′- O -Methyl-Oligoribonucleotide Complementary to U5 snRNA

10 May 1996

journal article
other
Published by American Association for the Advancement of Science (AAAS) in Science

Vol. 272 (5263) , 881-884
https://doi.org/10.1126/science.272.5263.881

Abstract

Nuclear messenger RNA splicing involves multiple interactions between the five spliceosomal small nuclear ribonucleoprotein particles (snRNPs) U1, U2, U4, U5, and U6 and numerous spliceosomal proteins. Here it is shown that binding of a 2′-O-methyl-oligoribonucleotide complementary to U5 small nuclear RNA (snRNA) nucleotides 68 to 88 (BU5Ae) disrupts the initial U4/U5/U6 tri-snRNP complex, enhances the U2/U6 interaction, and induces a U1/U4/U5 snRNP complex. The U1/U4/U5 snRNP complex interacts specifically with an RNA oligonucleotide containing the 5′ splice site sequence and may therefore represent a transitional stage in the displacement of U1 from the 5′ splice site by U5 snRNP.

Keywords

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