A Potent Transactivation Domain Mimic with Activity in Living Cells
- 18 May 2005
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 127 (23) , 8254-8255
- https://doi.org/10.1021/ja0515295
Abstract
Transcriptional coactivator-binding peptoids were isolated from a large combinatorial library. One of these molecules is shown to function as a potent activation domain surrogate in mammalian cells. Up to a 900-fold increase in expression of a Gal4-responsive reporter gene is observed when a steroid conjugate of the peptoid is incubated with HeLa cells expressing a Gal4 DNA-binding domain (DBD)−glucocorticoid receptor ligand-binding domain (GRLBD) fusion protein.Keywords
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