Two stages of the differentiation of HL-60 cells induced by 1α,25 dihydroxyvitamin D3; Commitment by 1α,25 dihydroxyvitamin D3 and promotion by DMSO
- 1 January 1986
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 6 (1) , 95-101
- https://doi.org/10.1007/bf01145184
Abstract
The differentiation of HL-60 cells induced by 1α,25 dihydroxyvitamin D3 was found to be separated into two stages, i.e. commitment and promotion. Most of the HL-60 cells were committed to monocyte/macrophage lineage by pretreatment with 1α,25 dihydroxyvitamin D3 (5–50 ng/ml) for 18–24 hr. The promotion in the second stage was inducer and lineage independent; treatment with 1.25% DMSO for 2 or 3 days promoted the differentiation of the committed HL-60 cells by 1α,25 dihydroxyvitamin D3 into monocyte/macrophage lineage, but not granulocyte lineage.Keywords
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