INDUCTION OF FOS RNA BY DNA-DAMAGING AGENTS

  • 1 April 1989
    • journal article
    • research article
    • Vol. 49  (7) , 1687-1692
Abstract
We have found that a wide variety of DNA-damaging agents and heat shock increase fos RNA to different extents in Chinese hamster ovary cells. These include the monofunctional alkylating agents methyl methanesulfonate and N-methyl-N''-nitro-N-nitrosoguanidine, the cross-linking agents cisPt(II) diamminedichloride and mechlorethamine HCl, the DNA base-damaging agents 4-nitroquinoline-N-oxide and N-acetoxy-2-acetylaminofluorene, ultraviolet and near-ultraviolet radiation, hydrogen peroxide, and Adriaymcin. Prolonged increases in fos RNA were observed, with persistence to 16 h following treatment. Variation in the amount of fos RNA induction by these agents and the diverse types and frequencies of cellular damage produced by them suggested that there might be several different mechanisms responsible for increased fos RNA.