DIFFERENCES BETWEEN STRUCTURAL DYNAMICS OF PLASMA-MEMBRANES OF NORMAL HAMSTER LYMPHOCYTES AND LYMPHOID-CELLS NEOPLASTICALLY TRANSFORMED BY SIMIAN VIRUS-40 AS REVEALED BY LASER RAMAN-SPECTROSCOPY
- 1 January 1977
- journal article
- research article
- Vol. 37 (10) , 3490-3493
Abstract
The Raman spectra of highly purified plasma membranes from SV40 transformed GD248 lymphocytes were compared with the spectra of the membranes of normal cells over the spectral region 100-3010 cm-1. Striking differences between the 2 membrane categories were observed in the thermal response of the CH stretching and acoustical regions. Analysis of CH stretching shows that the membranes of normal cells exhibit a thermal transition centered at 7.degree. C and .apprx. 5.degree. C wide. The membranes of GD248 cells show a lipid transition centered at -5.degree. C and 12-18.degree. C wide. Analysis of the acoustical region yields equivalent results. The membrane proteins of normal membranes undergo a large thermotropic transition, starting at 39.degree. C (sample temperatures), whereas this transition begins at 23.degree. C with GD248 plasma membranes. The results suggest the possibility that SV40 specific membrane proteins may modify the collective thermotropic behavior of normal membrane proteins and membrane lipids.This publication has 16 references indexed in Scilit:
- Erythrocyte membranes undergo cooperative, pH-sensitive state transitions in the physiological temperature range: evidence from Raman spectroscopy.Proceedings of the National Academy of Sciences, 1976
- Deuterated fatty acids as Raman spectroscopic probes of membrane structureBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
- Interaction of concanavalin A with rabbit thymocyte plasma membranes Distinction between low affinity association and positively cooperative binding mediated by a specific glycoproteinBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- Regulation of active alpha-aminoisobutyric acid transport expressed in membrane vesicles from mouse fibroblasts.Proceedings of the National Academy of Sciences, 1976
- Detergent solubilized and molecular weight estimation of tumor specific surface antigen from SV40 virus transformed cellsBiochemical and Biophysical Research Communications, 1976
- Sodium-stimulated alpha-aminoisobutyric acid transport by membrane vesicles from simian virus-transformed mouse cells.Proceedings of the National Academy of Sciences, 1976
- Multiple thermotropic state transitions in erythrocyte membranes a Laser-Raman study of the CH-stretching and acoustical regionsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- Sodium-stimulated amino acid uptake into isolated membrane vesicles from Balb/c 3T3 cells transformed by simian virus 40.Proceedings of the National Academy of Sciences, 1976
- Effect of melittin on thermotropic lipid state transitions in phosphatidylcholine liposomesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- Concanavalin a stimulation modifies the lipid and protein structure of rabbit thymocyte plasma membranes. A laser raman studyBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976