The Purification of Human Prothrombin

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Abstract
A method has been presented for the preparation of human prothrombin. The product is apparently homogenous by chromatographic, ultracentrifugal, electrophoretic and immunochemical analysis. It contains less than 1 part in 107 of thrombin and has a specific activity of 1560 u/mg dry weight. Problems related to the variability in specific activity of prothrombin preparations reported in the literature have been critically reviewed. * This investigation was supported in part by Research Grants from the National Science Foundation and National Institutes of Health and was carried out during tenure of National Heart Institute Special Fellowship No. 2-F3-HE-13, 942 (S. S. S.). ** Present address: Jefferson Medical College, Cardeza Foundation for Hematologic Research, 1015 Sansom Street, Philadelphia, Pa.