SUPEROXIDE DISMUTASE IN FACULTATIVELY ANAEROBIC BACTERIA: ENZYME LEVELS IN RELATION TO GROWTH CONDITIONS

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Abstract
Specific activities of superoxide dismutase [EC 1.15.1.1] (SOD) ranged from 9.3-18.3 U[units]/mg protein among 11 typical strains of facultatively anaerobic bacteria [Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Bacillus circulans, B. polymyxa and B. maceraus] grown under the same condition. All of the SOD in these bacteria were resistant to 1 mM KCN, suggesting the exclusion of Cu-Zn-SOD. Every bacterium examined had SOD resistant to 5 mM H2O2, suggesting MnSOD. Some had H2O2-sensitive SOD which were presumed to be Fe-SOD. Several cultural factors were examined in their effect on SOD activities in E. coli B, a typical facultative anaerobe. Aeration and manganese sulfate supplementation to media clearly affected both the levels and the acrylamide gel electrophoretograms of SOD. Aeration induced 2 of the 3 isoenzymes, the 3rd-SOD and Mn-SOD. Manganese sulfate promoted the induction of Mn-SOD by aeration.

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