In vitro model for the assessment of luminal factors on rectal mucosa.
Open Access
- 1 September 1983
- Vol. 24 (9) , 812-817
- https://doi.org/10.1136/gut.24.9.812
Abstract
An organ culture method for the maintenance of rectal biopsies over a period of 24 hours is described. Good preservation of histological architecture and continued crypt cell proliferation were shown over the culture period. The colonic enzyme alkaline phosphatase was found to rise over the period of culture. This rise was dependent upon continued protein synthesis by the cell. Changes in alkaline phosphatase activity during culture in biopsies from patients with ulcerative colitis and Crohn's disease are reported. This organ culture system and the measurement of alkaline phosphatase activity during culture provides a new approach to the assessment of luminal antigens as possible effectors of colonic epithelial cell damage.Keywords
This publication has 14 references indexed in Scilit:
- Rate and pattern of epithelial cell proliferation in ulcerative colitis.Gut, 1981
- Effect of prednisolone on prostaglandin synthesis by rectal mucosa in ulcerative colitis: investigation by laminar flow bioassay and radioimmunoassay.Gut, 1981
- Re-evaluation of the techique of organ culture for studying gluten toxicity in coeliac disease.Gut, 1978
- Short-term course and prognosis of Crohn's diseaseGut, 1974
- An in vitro model of gluten-sensitive enteropathy. Effect of gliadin on intestinal epithelial cells of patients with gluten-sensitive enteropathy in organ culture.Journal of Clinical Investigation, 1974
- Further studies of the kinetics of rectal epithelium in normal subjects and patients with ulcerative or granulomatous colitisDiseases of the Colon & Rectum, 1969
- THE DIFFERENTIATION OF ALKALINE PHOSPHATASE IN THE SMALL INTESTINEAnnals of the New York Academy of Sciences, 1969
- Kinetic studies of the epithelial cells of the rectal mucosa in normal subjects and patients with ulcerative colitis.Gut, 1966
- A new fluorometric method for RNA and DNA determinationAnalytical Biochemistry, 1966
- Cortisone in Ulcerative ColitisBMJ, 1955