Regulation of immune responses by I-J gene products. III. GT-specific suppressor factor is composed of separate I-J and idiotype-bearing chains.

Open Access

1 March 1983

journal article
research article
Published by Oxford University Press (OUP) in The Journal of Immunology

Vol. 130 (3) , 1274-1279
https://doi.org/10.4049/jimmunol.130.3.1274

Abstract

Injection of poly(Glu50Tyr50)(GT) into B10.BR (H-2k) mice induces GT-specific suppressor T cells and a T cell-derived suppressor factor (TsF1), which in turn induces a second-order suppressor T cell (TS2). In the present study, we show that B10.BR GT-TSF1 is composed of separate I-Jk and idiotype-bearing chains linked by disulfide bond(s). Functional suppressive activity requires both chains to be in association. Neither chain alone can induce TS2, indicating that both chains must be seen in association and suggesting a single cellular target for the two chains. Experiments designed to interchange I-J-bearing chains of GT-TSF1 derived from different H-2 haplotypes indicate that only the homologous I-J and idiotype-bearing chains can reassociate into a suppressive moiety. These experiments may imply heterogeneity of I-J region gene products.

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