Citrobacter O-antigens: structure of the O-antigenic polysaccharide from Citrobacter sp. 396

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Abstract
The structure of the O-specific polysaccharide moiety of the lipopolysaccharide from Citrobacter 396 was elucidated by composition, methylation and periodate oxidation studies. The repeating unit consists of 4 2-linked mannoses and 1 3-linked N-acetylglucosamine. One of the mannose units is substituted at C3 with .alpha.-glucose and 1 is substituted at C3 with .alpha.-(2-O-acetyl)-abequose. All the mannosyl linkages appear to have the .beta.-configuration; the N-acetylglucosaminyl linkage has the .alpha.-configuration. In bacterial agglutination and passive hemagglutination in some Salmonella antisera, Citrobacter 396 and its O-antigenic lipopolysaccharide expressed the serological factors 5 and 6. In corroboration of the structural studies, this showed the presence of .alpha.-(2-O-acetyl)-abequosyl-1,3-mannose (factor 5) and .alpha.-glucosyl-1,3-mannose (factor 6).