Studies of Chemotaxis in a Simple Chamber Including Observations Using Cells from Allergic Human Subjects and Their Allergens

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Abstract
Summary: A relatively simple method of observing granulocyte migration and chemotactic activity is described. It is a modification of the technique of Martin et al. (1) and it makes possible a permanent preparation for the observation of chemotaxis and the phenomena occurring when polymorphonuclear leukocytes meet bacteria or other objects placed in their path. Observations with this preparation are described. The response of the leukocytes to a strain of S. aureus, K. pneumoniae, and C. albicans were the same in bloods from all the subjects studied. This was true irrespective of positive or negative delayed skin reaction to the organism used in the preparations. With the strains of streptococcus and pneumococcus, observations were less constant. A halo appearance seen in some preparations but not others when chicken blood was used with fragments of Rous sarcoma is illustrated.

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