Soluble endoribonuclease activity from vaccinia virus: specific cleavage of virion-associated high-molecular-weight RNA
- 1 June 1978
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 26 (3) , 822-824
- https://doi.org/10.1128/jvi.26.3.822-824.1978
Abstract
A soluble endoribonuclease activity was extracted from purified vaccinia virus cores by treatment with sodium-deoxycholate and dithiothreitol. The soluble enzyme readily cleaved purified virion-associated high-molecular-weight RNA to limit-sized fragments sedimenting at 8 to 12S. Purified virion-released 8 to 12S polyadenylated mRNA was not degraded by the enzyme extract. The soluble endoribonuclease did not require the presence of ribonucleoside triphosphates for activity.This publication has 7 references indexed in Scilit:
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