In Vitro Propagation of Quercus shumardii Seedlings

Abstract
Single node stem sections of Quercus shumardii Buckl. seedlings were used to propagate Shumard oak in vitro. Stem sections in liquid woody plant medium (WPM) with 8.9 μm (2 mg liter−1) BA produced the greatest number of shoots ≥10 mm. The cytokinin 2iP did not promote axillary shoot growth. Shoots were rooted with 73% success under ex vitro conditions in Jiffy-7 peat pellets after a basal dip in 2.5 μm (0.5 g-liter−1) IBA. Plantlets were acclimatized successfully to greenhouse conditions. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA), N-(3-methyl-2-butenyl)-1H-purin-6-amine (2iP), 1H-indole-3-butanoic acid (IBA), (1α,2β,4aα,4bβ,10β)-2,4a,7-trihy- droxy-1-methyl-8-methylenegibb-3-ene-1,10-dicarboxylic acid 1,4a-lactone (GA3).

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