Antioxidative Activities of α-Tocotrienol and Its Derivative in the Oxidation of Dilinoleoylphosphatidylcholine Liposomes

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Abstract
The antioxidative activity of α-tocotrienol (α-Toc 3) and its derivative was studied in the 2, 2'-azobis (2-amidinopropane) dihydrochloride (AAPH) initiated oxidation of dilinoleoylphosphatidylcholine (DLPC) lipo-somes. Two DLPC concentrations, 1.93×10-3M for total aqueous dispersion (condition-I) and around 0.3×10-3M for total aqueous dispersion (condition-II), were used, keeping the molar concentrations of antioxidants or initiator for DLPC constant. A kinetic study indicated that in DLPC bilayers, α-Toc 3 had the same anti-oxidative activity as α-tocopherol (α-Toc) at both concentrations. However, α-Toc 3 showed higher antioxidative activity than α-Toc when was added after liposome formation. The synthesized water-soluble derivative of α-Toc 3 added after DLPC liposome formation under condition-II, as well as water-soluble carboxy-2, 5, 7, 8-tetramethyl-6-chromanol, showed about the same antioxidative activity as α-Toc 3 mixed with DLPC prior to liposome formation under condition-II. Solubility in water was a factor determining the antioxidative activity of the water-soluble antioxidant under condition-II. It would thus appear that α-Toc3 shows higher antioxidative activity than α-Toc in vitro in the synthetic liposome system, and that this can be explained on the basis of the distribution of α-Toc 3 in, or in the vicinity of the DLPC bilayers.

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