A KI-1 (CD30)-POSITIVE HUMAN CELL-LINE (KARPAS-299) ESTABLISHED FROM A HIGH-GRADE NON-HODGKINS LYMPHOMA, SHOWING A 2-5 TRANSLOCATION AND REARRANGEMENT OF THE T-CELL RECEPTOR BETA-CHAIN GENE

  • 1 July 1988
    • journal article
    • research article
    • Vol. 72  (1) , 234-240
Abstract
We describe the characterization of a new human cell line, Karpas 299 (K299), established from blast cells in the peripheral blood of a 25-year-old white man. His illness, which began with enlarged occipital and axillary nodes and weight loss, ended after 7 months with generalized lymphadenopathy, pleural effusion, and bone marrow involvement. A lymph node biopsy showed a large cell lymphoma mainly sinusoidal in distribution. The blast cells with pleomorphic nuclei resembled primitive histiocytes. The cells, which expresed the T-cell-associated markers CD4 and CD5, were positive for HLA-DR, epithelial membrane antigen, and CD30 (Ki-1 antigen). The karyotype was aneuploid and included a translocation 2;5. The site of translocation on chromosome 5 (at 5q35.1) is in the region of the locus of the c-fms oncogene (receptor of the monocyte-macrophaqe colony-stimulating factor MCSF or CSF-1). The cell line Karpas 299 has the same karyotype and pattern of antigen expression as the patient''s cells. Northern blot analysis of RNA showed an active rarrangement of the T-cell receptor .beta.-chain gene. This is to our knowledge the first Ki-1 antigen-positive line to be established from a case of non-Hodgkin''s lymphoma.