Cell fusion-impaired variants of a mouse lymphocytic cell line obtained by single-step selection for polyethylene glycol resistance
- 1 January 1980
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 6 (1) , 127-137
- https://doi.org/10.1007/bf01538701
Abstract
This report describes an effective method for single-step isolation of cell line variants with reduced intercellular plasma membrane fusion ability. Variants, derived from a subline of L5178Y, were obtained by selection for growth in the presence of a toxic level of PEG 6000. These variants occurred after EMS treatment and spontaneously. Further testing revealed a pleiotropic phenotype, fusion impairment and polyethylene glycol resistance, which has been designated PgfR. Variant sublines, in serial subculture for more than 165 days on nonselective medium, retain a stable PgfR phenotype. PgfR variants have an approximate 7-fold reduction in intercellular fusion competence.Keywords
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