Prevalence ofnimgenes in anaerobic/facultative anaerobic bacteria isolated in South Africa
Open Access
- 1 March 1999
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 172 (1) , 79-83
- https://doi.org/10.1111/j.1574-6968.1999.tb13453.x
Abstract
This study investigated the prevalence of nim genes (proposed to encode a 5-nitroimidazole resistance product) in 64 anaerobic/facultative anaerobic bacteria. Employing universal nim gene primers, 458-bp amplified fragments were recorded as presumptive positives in 22/64 strains at an annealing temperature of 52°C and 15/64 strains at 62°C, of which seven were propionibacteria. DNA sequencing confirmed the presence of nimA genes in Propionibacterium spp. (five strains), Actinomyces odontolyticus (one strain), Prevotella bivia (one strain) and Clostridium bifermentans (one strain) and nimB genes from five strains of Bacteroides fragilis. nimA genes were predominant in propionibacteria indicating a potential nimA gene source in anaerobic environments.Keywords
This publication has 13 references indexed in Scilit:
- Metabolism of a 5-nitroimidazole in susceptible and resistant isogenic strains of Bacteroides fragilisAntimicrobial Agents and Chemotherapy, 1997
- Antimicrobial Resistance in AnaerobesClinical Infectious Diseases, 1997
- Detection by PCR of the nim genes encoding 5-nitroimidazole resistance in Bacteroides sppJournal of Clinical Microbiology, 1996
- Plasmids plP419 and plP421 from Bacteroides: 5-nitroimidazole resistance genes and their upstream insertion sequence elementsMicrobiology, 1995
- Nucleotide sequence analysis of two 5-nitroimidazole resistance determinants from Bacteroides strains and of a new insertion sequence upstream of the two genesAntimicrobial Agents and Chemotherapy, 1994
- Nitroimidazole drugs-action and resistance mechanisms I. Mechanism of actionJournal of Antimicrobial Chemotherapy, 1993
- Cloning of aBacteroides fragilischromosomal determinant coding for 5-nitroimidazole resistanceFEMS Microbiology Letters, 1992
- Genetic and molecular analysis of pIP417 and pIP419:Bacteroides plasmids encoding 5-nitroimidazole resistancePlasmid, 1992
- Inactivatin of metronidazole by Enterococcus faecalisJournal of Antimicrobial Chemotherapy, 1991
- Reductive activation of nitroimidazoles in anaerobic microorganismsBiochemical Pharmacology, 1986