FORMATION OF IMMUNOGLOBULINS BY HUMAN TISSUES IN VITRO .2. QUANTITATIVE STUDIES

  • 1 January 1966
    • journal article
    • research article
    • Vol. 11  (1) , 13-+
Abstract
The present paper deals with some of the quantitative aspects of immunoglobulin formation in vitro. The results of this study show that isolated spleen cells synthesize about 1. 25 [mu]g IgG per 108 lymphoid cells during the 1st 6 hrs. of incubation. The initial rate of synthesis was not maintained throughout the 48 hrs. of incubation, but decreased markedly after 6 hrs. Comparison of the intensity of the autoradiographic line with the amount of radioactive immunoglobulin in the precipitation line revealed that a very distinct line (gradation +++) corresponds to about 0. 0125 [mu]g IgG. The limit of visibility (+) is reached at approximately 0. 0002 [mu]g radioactive IgG.