Correlation between hypo-osmotic swelling test and ‘water test’ to assess human sperm membrane integrity

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Abstract
Fifty-nine men who requested vasectomy and 43 infertile patients had a semen analysis performed prior to surgery or during evaluation. A hypo-osmotic swelling test (HOS) and a new 'Water test' were performed simultaneously, in order to assess correlation between these two procedures. Our results showed that values obtained with the 'Water test' were significantly higher than those obtained with the HOS test (P < 0.001). These findings suggest that it is necessary to determine normal values for this new test before introducing it in the routine semen analysis.