Inhibition of Simian Virus 40 DNA replication in CV-1 cells by an oligodeoxynucleotide covalently linked to an intercalating agent
- 31 December 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (10) , 2901-2908
- https://doi.org/10.1093/nar/18.10.2901
Abstract
An octathymidylate covalently linked via its 3''-end to an acridine derivative inhibited the cytopathic effect of Simian Virus SV40 on CV-1 cells in culture. Centrol experiments revealed that this effect was virus-specific and did not arise as a result of oligonucleotide degradation by nucleases. A photoactive probe was covalently attached to the 5''-end of the oligonucleotide-acridine conjugate. Upon UV-irradiation, photocrosslinking was shown to occur at the A.T-rich region within the viral origin of replication. A local triple helix can form at moderate salt concentrations with two octathymidylate-acridine conjugates bound to the octaadenylate sequence. Alternatively the octathymidylate-acridine conjugate can bind to the major groove of duplex DNA forming a local triple helix. Different mechanisms are discussed to explain the inhibition of viral DNA replication.This publication has 45 references indexed in Scilit:
- Rate of degradation of [α]- and [β]-oligodeoxynucleotides inXenopusoocytes. Implications for anti-messenger strategiesNucleic Acids Research, 1987
- Anti-messenger oligodeoxynucleotides: specific inhibition of rabbit β-globin synthesis in wheat germ extracts and Xenopus oocytesBiochimie, 1986
- The use of single-stranded DNA and RNase H to promote quantitative ‘hybrid arrest of translation’ of mRNA/DNA hybrids in reticulocyte lysate cell-free translationsNucleic Acids Research, 1986
- THE ROLE OF ANTISENSE RNA IN GENE REGULATIONAnnual Review of Biochemistry, 1986
- Inhibition of replication and expression of human T-cell lymphotropic virus type III in cultured cells by exogenous synthetic oligonucleotides complementary to viral RNA.Proceedings of the National Academy of Sciences, 1986
- Antiviral effect of an oligo(nucleoside methylphosphonate) complementary to the splice junction of herpes simplex virus type 1 immediate early pre-mRNAs 4 and 5.Proceedings of the National Academy of Sciences, 1986
- Specific inhibition of mRNA translation by complementary oligonucleotides covalently linked to intercalating agents.Proceedings of the National Academy of Sciences, 1986
- Translation arrest by oligodeoxynucleotides complementary to mRNA coding sequences yields polypeptides of predetermined lengthNucleic Acids Research, 1986
- Hybridization arrest of globin synthesis in rabbit reticulocyte lysates and cells by oligodeoxyribonucleoside methylphosphonatesBiochemistry, 1985
- Oligodeoxynucleotides covalently linked to intercalating agents: a new class of gene regulatory substancesBiochimie, 1985