RECEPTORS FOR MULTIPLICATION-STIMULATING ACTIVITY ON HUMAN ARTERIAL AND VENOUS ENDOTHELIAL CELLS

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Abstract
Primary cultures of endothelial cells were prepared from the arteries and veins of human umbilical cords. Both arterial and venous endothelial cells demonstrated specific receptors for the insulin-like growth factor MSA (Multiplication-Stimulating Activity). Insulin, at concentrations up to 10−7M, had little effect on 125I-MSA binding whereas MSA was =1% as potent as insulin in competing with 125I-insul in binding. Serum containing anti-insulin receptor antibodies blocked binding of 251-insulin to the endothelial cells but had little effect on 125I-MSA binding. We conclude that human endothelial cells have specific and distinct receptors for both MSA and insulin.

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