Cultural and Other Morphological Studies of Sparassis radicata and Related Species

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Abstract
S. radicata Weir was found to be heterothallic with a bipolar type of mating system. Multiple alleles for incompatibility occur at the locus for heterothallism. Tests were negative for the production of extracellular oxidases on gallic or tannic acid media and with gum guaiac solution. The optimum temperature for growth on 3.0% malt extract agar medium was 23-25.degree. C. Studies of some basidiocarps and cultures labeled S. crispa Wulf. ex Fr. from Europe and Japan show they are conspecific with S. radicata. Specimens and isolates labeled S. crispa from SE USA differed from the European and Japanese specimens and from isolates labeled S. crispa. Apparent dikaryotization of single basidiospore isolates of S. radicata from Arizona, USA, by dikaryotic isolates labeled S. cripsa from Europe and Japan offers additional evidence for the conspecificity of these fungi. Descriptions of basidiocarps and cultural characters are given for S. radicata and for specimens and cultures labeled S. crispa.

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