A modified Kelsey‐Sykes method for testing disinfectants with 2,3,5‐triphenyltetrazolium chloride reduction as an indicator of bacterial growth
- 1 June 1987
- journal article
- research article
- Published by Wiley in Journal of Applied Bacteriology
- Vol. 62 (6) , 551-554
- https://doi.org/10.1111/j.1365-2672.1987.tb02688.x
Abstract
Different dilutions of chlorhexidine gluconate were tested by the Kelsey-Sykes procedure. The method was further modified on microtitration plates using 2,3,5-triphenyltetrazolium chloride (TTC) reduction as an indicator of bactrial growth. There was a good correlation with the results based on TTC reduction and the conventional method based on turbidity changes caused by bacterial growth. Furthermore, the modified method using TTC reduction is more rapid and can be read by the naked eye because of the red colour.This publication has 2 references indexed in Scilit:
- The Principles involved in the Standardisation of Disinfectants and the Influence of Organic Matter upon Germicidal valueEpidemiology and Infection, 1908
- Standardisation of DisinfectantsJournal of the Sanitary Institute, 1903