Comparison of Abilities of Salmonella enterica Serovar Typhimurium aroA aroD and aroA htrA Mutants To Act as Live Vectors
Open Access
- 1 October 2000
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 68 (10) , 6041-6043
- https://doi.org/10.1128/iai.68.10.6041-6043.2000
Abstract
We compared the ability of Salmonella enterica serovar Typhimurium SL1344 aroA aroD (BRD509) and aroA htrA (BRD807) mutants to act as live vectors for delivery of fragment C of tetanus toxin (FrgC). FrgC was expressed in these strains from either pTETnir15 or pTEThtrA1. BRD509FrgC + strains elicited ∼2-log-higher serum anti-FrgC antibody titers than BRD807FrgC + strains. All mice immunized with BRD807pTEThtrA1, BRD509pTEThtrA1, and BRD509pTETnir15 (but not BRD807pTETnir15) were protected against tetanus.Keywords
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