Tumor Antigen Pulsed Dendritic Cells Enhance the Cytolytic Activity of Tumor Infiltrating Lymphocytes in Human Hepatocellular Cancer

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Abstract
Objective Tumor infiltrating lymphocytes (TILs) stimulated with interleukin —2 (IL-2) ex vivo have been successfully used therapeutically in some cancer patients, but their potency in eliciting an effective anti-tumor response is variable. We have tried to augment killing activity of tumor infiltrating lymphocytes derived from hepato- cellular carcinoma (HCC) using autologous monocytes derived dendritic cells. Methods Tumor infiltrating lymphocytes (TILs) from 6 patient with hepatocellular carcinoma were isolated and the phenotype were further characterized. From the same patients, autologous dendritic cells were generated from CD 14+ monocytes that were cultured for 6 days in the presence of granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4). Those professional antigen presenting cells were pulsed with whole autologous hepatoma tumor lysates (pDC). TILs were cocultured with pDC or unpulsed DC. To assess the cytotoxic potency of TILs, the ability to lyse the tumor cell targ...