LIPASE AND ACETYL ESTERASE ACTIVITIES IN INTACT BACILLUS COAGULANS SPORES
- 1 June 1966
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 44 (6) , 677-685
- https://doi.org/10.1139/o66-085
Abstract
Since data on esterase content of intact dormant spores are meager, a study was carried out with various enzyme substrates and several methods of product detection on intact and ruptured preparations of Bacillus coagulans. Measurement of hydrolysis of triacetin, naphthyl acetates, fluorescein diacetate, ethyl acetate, and ethyl butyrate demonstrated the presence of arylesterase, carboxylesterase, acetyl esterase, and lipase activities in both the intact and ruptured preparations of the non-germinated organism used. It was observed that spore esterase was inhibited by normal and basic copper acetate, eserine, taurocholate, 253.7 mμ ultraviolet light, and temperatures between 50 and 100 °C.Keywords
This publication has 1 reference indexed in Scilit:
- Electrophoretic Heterogeneity of TrypsinNature, 1959