Quantification of total T-cell receptor diversity by flow cytometry and spectratyping
Open Access
- 6 August 2013
- journal article
- Published by Springer Nature in BMC Immunology
- Vol. 14 (1) , 35
- https://doi.org/10.1186/1471-2172-14-35
Abstract
T-cell receptor diversity correlates with immune competency and is of particular interest in patients undergoing immune reconstitution. Spectratyping generates data about T-cell receptor CDR3 length distribution for each BV gene but is technically complex. Flow cytometry can also be used to generate data about T-cell receptor BV gene usage, but its utility has not been compared to or tested in combination with spectratyping.Keywords
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