Form of Conjugation of Steroids in Urine and in the Adrenal Glands of a Patient with Cushing's Syndrome Owing to Adrenal Cortical Hyperplasia and a Basophilic Adenoma of the Pituitary Gland

Abstract
Several steroids in the urine and adrenal tissue of a boy with Cushing's syndrome and a basophilic adenoma of the pituitary gland have been studied. The outstanding observation was that large quantities of free cortisol were excreted; a considerable amount of this hormone was also found in the adrenal tissue. There appeared to be deficiencies in the mechanisms for conjugation with glucuronic acid, for the reduction of the Δ4-3-keto grouping and for the dehydrogenation of 11β-hydroxysteroids. In this paper we also report the identification and isolation of 6β,17α,21-triol-4-pregnene-3,11,20-trione (6β-hydroxycortisone).

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