Isolation and Analysis of Somatic Hybrids Derived from Two Human Diploid Cells
- 1 March 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (3) , 937-941
- https://doi.org/10.1073/pnas.71.3.937
Abstract
Lesch-Nyhan fibroblasts and normal human leukocytes with different glucose-6-phosphate dehydrogenase genotypes were fused by Sendai virus. Clones were isolated on the basis of their resistance to a medium containing hypoxanthine, amethopterin, and thymidine and ability to proliferate in monolayer culture. These mononuclear cells ( 1 ) incorporated [ 3 H]hypoxanthine, ( 2 ) expressed the glucose-6-phosphate dehydrogenase heteropolymer, and ( 3 ) were polyploid. Therefore, hybrids can originate from the fusion of two diploid human cells. X chromosome inactivation did not occur in these hybrid cells of male origin. The hybrids expressed both parental genomes and exhibited the senescence and contact feeding characteristic of the human skin fibroblast.Keywords
This publication has 16 references indexed in Scilit:
- Evidence for two functional X chromosomes in human oocytesCell Differentiation, 1972
- Stability of X Chromosomal Inactivation in Human Somatic CellsNature, 1972
- Communication between normal and enzyme deficient cells in tissue cultureExperimental Cell Research, 1972
- Parasexual Cycle in Cultivated Human Somatic CellsScience, 1969
- HYBRIDIZATION OF TWO BIOCHEMICALLY MARKED HUMAN CELL LINESProceedings of the National Academy of Sciences, 1969
- EVIDENCE FOR INTERGENIC COMPLEMENTATION IN HYBRID CELLS DERIVED FROM TWO HUMAN DIPLOID STRAINS EACH CARRYING AN X-LINKED MUTATIONProceedings of the National Academy of Sciences, 1969
- X-Linked Hypoxanthine-Guanine Phosphoribosyl Transferase Deficiency: Heterozygote Has Two Clonal PopulationsScience, 1968
- VARIATION OF GLUCOSE-6-PHOSPHATE DEHYDROGENASE IN DIFFERENT POPULATIONSThe Lancet, 1964
- DEMONSTRATION OF TWO POPULATIONS OF CELLS IN THE HUMAN FEMALE HETEROZYGOUS FOR GLUCOSE-6-PHOSPHATE DEHYDROGENASE VARIANTSProceedings of the National Academy of Sciences, 1963
- Sex Chromatin Patterns and the Lyon HypothesisScience, 1963