Differences in the Ligand Specificity between CD1d‐Restricted T Cells with Limited and Diverse T‐Cell Receptor Repertoire

Publisher Website
Google Scholar
Abstract
The natural killer (NK) T‐lymphocyte population consists of two subsets utilizing a diverse and restricted T‐cell receptor (TCR) repertoire, respectively. Both populations have been shown to include autoreactive cells. NKT cells carrying restricted Vα14(AV14S1)Jα281/Vβ8.2(BV8S2A1) TCR have been shown to recognize α‐galactosylceramide (αGalCer) presented in the context of murine CD1d. In this study we screened a set of murine CD1d‐autoreactive T‐cell hybridomas with diverse TCR for their reactivity with several glycosylated variants of ceramide, including αGalCer. These hybridomas showed a different pattern of reactivity to CD1d‐expressing antigen‐presenting cells (APC) and were not reactive with any of the tested variants of ceramide. A second set of hybridomas had been selected for expression of Vα14 and Vβ8.2 TCR chains. These cells responded to αGalCer presented on CD1d, but were only weakly reactive to syngeneic splenocytes or CD1d‐transfected cells. Their fine specificity in the response to glycosylation variants of ceramide demonstrated a homogenous reactivity pattern, including reactivity to α‐galactosylsphingosine, the variant of αGalCer with truncated fatty acyl chain. These findings underline the differences in ligand specificity between the two subsets of CD1d‐restricted NKT cells, and demonstrate a similarity in reactivity among the hybridomas using the Vα14‐Jα281/Vβ8.2 TCR.

This publication has 0 references indexed in Scilit: