Expression in Bacillus subtilis of the gene for human urogastrone using synthetic ribosome binding sites

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Abstract
A chemically synthesised gene coding for human urogastrone which was earlier cloned in E. coli (Smith et al. 1982) has now been cloned into expression vectors for Bacillus subtilis Two types of constructs have been made, one giving production of methionylurogastrone and the other giving rise to a methionyl-urogastrone-β galactosidase fusion polypeptide facilitating quantification of expression levels. The ribosome binding sites used in the expression plasmids are synthetically made oligonucleotides residing on short restriction fragments to allow easy replacement by other ribosome binding sites. Using “shuttle” vectors and constitutive promoters from Bacillus phages π105 and SPP1, we were able to detect levels of expression amounting to a few thousand molecules per cell during logarithmic growth in both E. coli and B. subtilis.