Pigment Glands of Cottonseed. III. Distribution and Some Properties of Cottonseed Pigments

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Abstract
Samples of flaked or ground cottonseed kernels and cottonseed pigment glands, separated by the gland flotation process, were extracted with a number of different solvents for detn. of their pigment content. In some cases the solvents and materials to be extracted were dried to prevent rupture of the pigment glands. On the basis of absorption spectra of chloroform solns. of these extracts and their antimony trichloride reacton products, it was concluded that all of the pigments, gossypol and gossypurpurin, were segregated in the glands, and that these are the only detectable pigments in the pigment glands of the 4 vars. of Gossypium hirsutum examined. The gossypol content of the glands was found to be relatively constant .(approx. 50%); the gossypurpurin content was relatively low and variable. A yellow pigment has been detected in soln. in the oil of the extraglandular tissue of the kernel and has been obtained in soln. free of gossypol, gossypurpurin, and the yellow decomposition product of gossypurpurin. Partial characterization of this pigment has shown it to be relatively stable and to differ from every pigment previously detected in cottonseed. On the basis of the distribution and properties of the pigments of cottonseed, methods for their extraction and estimation were developed.

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