HORMONAL IMPRINTING IN CELL-CULTURE .1. IMPACT OF A SINGLE EXPOSURE TO INSULIN ON CELLULAR INSULIN BINDING-CAPACITY IN PERMANENT CELL-LINES

  • 1 January 1984
    • journal article
    • research article
    • Vol. 64  (1) , 57-63
Abstract
Three permanent cell lines showed a durable change in cellular insulin binding capacity in response to a single exposure to insulin. Initial increase in binding of FITC [fluorescein-isothiocyanate]-labeled insulin to both cytoplasmic and nuclear membrane was followed by a lasting decrease in the case of fibroblast (NCTC) and return to approximately the control level in epithelial (Chang liver) and tumor (HeLa) cell cultures. While the amplifying effect of a single 4-h exposure was measurable as soon as after 24 h, that of a single 24-h exposure came into display only after 72 h in the epithelial and tumor cell cultures. The latter 2 lines showed return of the binding capacity to the control value and a drop of it to a minimum 20 days after exposure for 4 and 24 h, respectively. Evidently, although the degree and manner of insulin binding differ between cell lines, the impact of a single exposure to insulin is durably felt by all.