Intracerebral injection of human immunodeficiency virus type 1 coat protein gp120 differentially affects the expression of nerve growth factor and nitric oxide synthase in the hippocampus of rat.
- 23 January 1996
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 93 (2) , 928-933
- https://doi.org/10.1073/pnas.93.2.928
Abstract
We have studied the neuropathological characteristics of the brain of rats receiving daily intracerebroventricular administration of freshly dissolved human immunodeficiency virus type 1 recombinant protein gp120 (100 ng per rat per day) given for up to 14 days. Histological examination of serial brain sections revealed no apparent gross damage to the cortex or hippocampus, nor did cell counting yield significant neuronal cell loss. However, the viral protein caused after 7 and 14 days of treatment DNA fragmentation in 10% of brain cortical neurons. Interestingly, reduced neuronal nitric oxide synthase (NOS) expression along with significant increases in nerve growth factor (NGF) were observed in the hippocampus, where gp120 did not cause neuronal damage. No changes in NGF and NOS expression were seen in the cortex, where cell death is likely to be of the apoptotic type. The present data demonstrate that gp120-induced cortical cell death is associated with the lack of increase of NGF in the cerebral cortex and suggest that the latter may be important for the expression of neuropathology in the rat brain. By contrast, enhanced levels of NGF may prevent or delay neuronal death in the hippocampus, where reduced NOS expression may be a reflection of a subcellular insult inflicted by the viral protein.Keywords
This publication has 29 references indexed in Scilit:
- HIV-1 gp120 Produces DNA Fragmentation in the Cerebral Cortex of RatBiochemical and Biophysical Research Communications, 1995
- Co-localization of nitric oxide synthase and NGF receptor in neurons in the medial septal and diagonal band nuclei of the ratNeuroscience Letters, 1994
- Lithium and Tacrine Increase the Expression of Nitric Oxide Synthase mRNA in the Hippocampus of RatBiochemical and Biophysical Research Communications, 1993
- The Envelope Glycoprotein of HIV‐1 Alters NMDA Receptor FunctionEuropean Journal of Neuroscience, 1993
- Neurotoxicity of HIV coat protein gp120, NMDA receptors, and protein kinase C: A study with rat cerebellar granule cell culturesJournal of Neuroscience Research, 1993
- Multiple sclerosis patients express increased levels of β-nerve growth factor in cerebrospinal fluidNeuroscience Letters, 1992
- Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation.The Journal of cell biology, 1992
- gp120 of HIV-1 induces apoptosis in rat cortical cell cultures: prevention by memantineEuropean Journal of Pharmacology: Molecular Pharmacology, 1992
- Models of neuronal injury in AIDS: another role for the NMDA receptor?Trends in Neurosciences, 1992
- Localization of nitric oxide synthase indicating a neural role for nitric oxideNature, 1990