Effect of autoxidized methyl linoleate on glutathione peroxidase.

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Abstract
Effect of several oxidation stages of autoxidized methyl linoleate (AOML) on glutathione peroxidase (GSH-Px) was studied. In the 1st experiment, GSH-Px was prepared from gastrointestinal tract and liver which were excised from mice and effects of AOML rich in hydroperoxides (HP) and that rich in decomposition products of HP on activity of GSH-Px were compared. GSH-Px indiscriminately metabolized both types of AOML without strict recognition of the specificity of the substrate. In the 2nd experiment, inhibitory rate of GSH-Px by in vitro incubation with various AOML closely correlated with POV [peroxide value] of sample esters but did not correspond with toxicity revealed by i.p. injection or intragastric feeding. In the next experiment, mice were given AOML with a low vitamin E diet for 45 days. Increased GSH-Px activity in the gastrointestinal tract in proportion to POV of administered oil occurred, but level in liver remained unchanged. Increased GSH-Px activity by i.p. injection of AOML in mice was marked in liver, but not in gastrointestinal tract. Most of the orally administered AOML was reduced in the gastrointestinal tract mucosa. Marked increase of fluorescence in lipid fraction of heart and kidney by oral administration of AOML suggested that damage caused by it was not limited to gastrointestinal tract but spread through the entire body.