Enzymatic Oligomerization of Bacteriophage P22 DNA and of Linear Simian Virus 40 DNA
- 1 November 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (11) , 3389-3393
- https://doi.org/10.1073/pnas.69.11.3389
Abstract
Linear double-stranded molecules of the circularly permuted and terminally redundant DNA of Salmonella bacteriophage P22 have been converted to oligomeric products in the presence of polynucleotide ligase coded for by the coliphage T4. The reaction has been monitored by sucrose density-gradient centrifugation and electron microscopy. It goes slowly and gives yields of 30-40%. The products are mainly dimers and trimers, but higher oligomers are also present.DNA ligase extracted from uninfected Escherichia coli seems unable to perform a similar reaction, which is concluded to involve the fully base-paired termini. Linear double-stranded molecules of simian virus(SV) 40 DNA, produced by the action of the bacterial restriction endonuclease R(1), are oligomerized by either ligase; therefore, this reaction seems to involve single-stranded cohesive ends. No mixed products could be found when P22 DNA and linear SV 40 DNA were exposed together to the T4 ligase.Keywords
This publication has 17 references indexed in Scilit:
- Polynucleotide Ligase-Catalyzed Joining of Deoxyribo-oligonucleotides on Ribopolynucleotide Templates and of Ribo-oligonucleotides on Deoxyribopolynucleotide TemplatesProceedings of the National Academy of Sciences, 1970
- Total Synthesis of the Gene for an Alanine Transfer Ribonucleic Acid from YeastNature, 1970
- Enzymatic breakage and joining of deoxyribonucleic acid. VI. Further purification and properties of polynucleotide ligase from Escherichia coli infected with bacteriophage T4.1968
- Studies on polynucleotides. LXXXVII. The joining of short deoxyribopolynucleotides by DNA-joining enzymes.Proceedings of the National Academy of Sciences, 1968
- The Intracellular Forms of T7 and P22 DNA MoleculesCold Spring Harbor Symposia on Quantitative Biology, 1968
- Cyclization of Phage DNAsPublished by Cold Spring Harbor Laboratory ,1968
- Polynucleotide cellulose as a substrate for a polynucleotide ligase induced by phage T4Biochemical and Biophysical Research Communications, 1967
- The enzymatic repair of DNA. I. Formation of circular lambda-DNA.Proceedings of the National Academy of Sciences, 1967
- Enzymatic joining of DNA strands: a novel reaction of diphosphopyridine nucleotide.Proceedings of the National Academy of Sciences, 1967
- Enzymatic breakage and joining of deoxyribonucleic acid, I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage.Proceedings of the National Academy of Sciences, 1967