CULTIVATION OF FASTIDIOUS MYCOPLASMAS FROM HUMAN ARTHRITIS

  • 1 January 1983
    • journal article
    • research article
    • Vol. 42  (2) , 66-69
Abstract
Joint fluid specimens (14) from arthritic patients and synovial tissue specimens (8) from traumatic joint lesions were studied for mycoplasmas. Fastidious slow-growing mycoplasmas were cultivated from arthritic patients, but not from the controls. Using a large field microscope and a lens correcting the thickness of the Dienes-stained agar block preparations, tiny granular colonies with a diameter of 15-50 .mu.m were seen. The isolates were arginine-positive but glucose- and urea-negative. In growth inhibition tests, there was a partial inhibition zone of 1-10 mm by Mycoplasma hominis type 2 and M. arthritidis immune sera. Thus, the isolates resembled mycoplasmas previously recovered from acute and chronic human arthritis in Finland.

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