Density‐gradient‐sedimentation velocity of solvated macromolecules: Theoretical considerations about the buoyancy factor

Publisher Website
Google Scholar
Abstract
The solvation of macromolecules lowers their sedimentation velocity in a density gradient. Some consequences of this effect are pointed out. Overlooking the solvation results in the understimation of the standard sedimentation coefficient of the macromolecules. (The error increases with partial specific volume, solvation, and the density in the gradient.) The construction of an isokinetic density gradient also requires that solvation be taken into account. This can be done when the solvation parameter, relevant in this context, is known.

This publication has 28 references indexed in Scilit: