Resistance to diethofencarb (NPC) in benomyl‐resistant field isolates of Botrytis cinerea

Abstract
Based on the ‘negative cross‐resistance’ phenomenon between N‐phenylcarbamates and benzimidazole fungicides, the fungicidal mixture of carbendazim + diethofencarb (MBC + NPC) was introduced, to control Botrytis cinerea phenotypes which are either sensitive to benzimidazoles and resistant to NPC (wild‐type: BenS NPCR). or resistant to benzimidazoles and sensitive to NPC(BenR NPCS). At one out of four sites where the MBC + NPC mixture was used in commercial cucumber greenhouses, grey mould control failed and a new phenotype of B. cinerea was found. The new phenotype was resistant to benzimidazoles, as was 100% of the population screened in the four sites, but retained resistance to NPC. Accordingly, the new phenotype was designated BenR NPCR. It was pathogenic on cucumber seedlings.

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