Micro-Radioimmunoassay for Antibodies to Newcastle Disease Virus in the Chicken
- 1 January 1975
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 114 (1_Part_2) , 422-425
- https://doi.org/10.4049/jimmunol.114.1_part_2.422
Abstract
An indirect micro-radioimmunoassay is described in which chicken anti-Newcastle disease virus antibody was detected, with radioactively labeled rabbit anti-chicken Fab, on virus-infected microcultures of chick embryo fibroblasts. Newcastle disease virus-infected microcultures were formalin fixed and stored at 4°C for up to 4 months without affecting the sensitivity of the test. The micro-technique was found to be a highly sensitive and specific assay of anti-viral antibody and may allow detection of immunoglobulin class of anti-Newcastle disease virus antibody.This publication has 0 references indexed in Scilit: