Evidence for the existence of an Ns-type regulatory protein in Trypanosoma cruzi membranes
- 1 August 1986
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 237 (3) , 913-917
- https://doi.org/10.1042/bj2370913
Abstract
The existence of a GTP-binding protein of the Ns type in Trypanosoma cruzi was explored. Epimastigote membranes were labelled by cholera toxin in the presence of [adenine-14C]NAD+. After SDS/polyacrylamide-gel electrophoresis of extracted membrane proteins, a single labelled polypeptide band of apparent Mr approx. 45,000 was detected. Epimastigote cells were treated with N-ethylmaleimide and electrofused to lymphoma S49 cells lacking the Ns protein. Evidence indicates that in such electrofusion-generated cell hybrids a heterologous adenylate cyclase system was reconstituted with the Ns protein provided by T. cruzi epimastigotes.This publication has 15 references indexed in Scilit:
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