Determination of α-Amanitin in Serum by HPLC
- 1 March 1983
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 6 (3) , 551-558
- https://doi.org/10.1080/01483918308076067
Abstract
A reverse-phase high-performance liquid chromatographic method for the quantitative determination of α-amanitin in serum of poisoned patients is described and discussed. The methodology developed is simple and rapid with a minimum of sample preparation steps required. Detection is very sensitive, allowing quantitation of 25 ng of α-amanitin/ml of serum. The technique described is a useful tool to determine the severity of Amanita phalloides intoxication during the first 24 h after ingestion of poisonous mushrooms.Keywords
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