A NOTE ON THE PURIFICATION OF FOLLICLE-STIMULATING AND LUTEINIZING HORMONES FROM BABOON PITUITARIES

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Abstract
Procedures for purification of human luteinizing hormone (LH) and human folliclestimulating hormone (FSH) (Stockell Hartree, 1966; Roos, 1968) have been applied to acetone-preserved baboon pituitaries. After death of the baboons in Kenya pituitaries were removed and placed in excess cold acetone. The glands were transported to Ohio where an acetone-dried powder was prepared, the yield from 185 pituitaries was 10·1 g. This material was extracted with 6% ammonium acetate at pH 5·1 in 40% ethanol and protein was precipitated from the soluble extract by addition of ethanol to 80% (GTN ppt). This fraction was then chromatographed on a column of CM-cellulose as described previously (Stockell Hartree, 1966). The nonadsorbed fraction (CM-1) was pooled, concentrated by rotary evaporation and freeze-dried. The adsorbed fraction (CM-2) was eluted with 1 m-ammonium acetate, and was precipitated with 80% ethanol. Fraction CM-2 (44 mg) was chromatographed on a column of DEAE-cellulose (Whatman DE-23, 1

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