VIABILITY AND FERTILITY OF DROSOPHILA EXPOSED TO SUB-ZERO TEMPERATURES
- 1 October 1949
- journal article
- research article
- Published by University of Chicago Press in The Biological Bulletin
- Vol. 97 (2) , 150-157
- https://doi.org/10.2307/1538294
Abstract
Observations on the viability and fertility of D. melanogaster after exposure to low temps. during the course of combination low temp. - X-irra-diation treatments indicate that at -5[degree]C about 50% mortality is reached after 2 hrs.; at -10[degree]C a 20 min. exposure kills very few, whereas a 25 min. exposure is almost completely lethal; at -15[degree]C about 50% survive exposures less than 10 min. long; at -20[degree]C all individuals are killed within a few min. It was found further that cold air shocks at -5[degree]C for 75 to 90 min. and at -10[degree] C for 5 to 20 min. are lethal to sperm stored in females although such treatment has no effect on the subsequent fertility of such females. This has proved to be of considerable value in expts. where large numbers of virgin females are needed since fertilized females may be "deseminated" by treatment with a low temp.Keywords
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