NAD+‐dependent DNA ligases of Mycobacterium tuberculosis and Streptomyces coelicolor
- 8 April 2003
- journal article
- research article
- Published by Wiley in Proteins-Structure Function and Bioinformatics
- Vol. 51 (3) , 321-326
- https://doi.org/10.1002/prot.10361
Abstract
Sequencing of the genomes of Mycobacterium tuberculosis H37Rv and Streptomyces coelicolor A3(2) identified putative genes for an NAD+-dependent DNA ligase. We have cloned both open reading frames and overexpressed the protein products in Escherichia coli. In vitro biochemical assays confirm that each of these proteins encodes a functional DNA ligase that uses NAD+ as its cofactor. Expression of either protein is able to complement E. coli GR501, which carries a temperature-sensitive mutation in ligA. Thus, in vitro and in vivo analyses confirm predictions that ligA genes from M. tuberculosis and S. coelicolor are NAD+-dependent DNA ligases. Proteins 2003;51:321–326.Keywords
This publication has 25 references indexed in Scilit:
- Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)Nature, 2002
- Conserved Residues in Domain Ia Are Required for the Reaction of Escherichia coli DNA Ligase with NAD+Journal of Biological Chemistry, 2002
- NAD+-dependent DNA Ligase Encoded by a Eukaryotic VirusJournal of Biological Chemistry, 2001
- A family of DNA repair ligases in bacteria?FEBS Letters, 2001
- Bacterial DNA ligasesMolecular Microbiology, 2001
- Cloning and Functional Characterization of an NAD + -Dependent DNA Ligase from Staphylococcus aureusJournal of Bacteriology, 2001
- Gleaning non-trivial structural, functional and evolutionary information about proteins by iterative database searchesJournal of Molecular Biology, 1999
- Functional domains of an NAD + -dependent DNA ligase 1 1Edited by A. R. FershtJournal of Molecular Biology, 1999
- Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequenceNature, 1998
- RNA capping enzyme and DNA ligase: a superfamily of covalent nucleotidyl transferasesMolecular Microbiology, 1995