Ca2+, calmodulin‐dependent phosphorylation of glycogen synthase by a brain protein kinase
- 5 September 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 161 (1) , 28-32
- https://doi.org/10.1016/0014-5793(83)80723-6
Abstract
A Ca2+, calmodulin-dependent protein kinase from brain with a M r of 640 000 is capable of phosphorylating glycogen synthase from skeletal muscle. The reaction was inhibited by the addition of 1 mM EGTA and 50 μM trifluoperazine, but not by protein kinase inhibitor and heparin. The amount of phosphate incorporated into glycogen synthase was 1.4 mol/mol subunit. The phosphorylation sites of glycogen synthase were cyanogen bromide-treated peptides CB-1 and CB-2 and only the seryl residue was phosphorylated.Keywords
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